Composite
Part:BBa_K4463004:Design
Designed by: Qiaowa Gong Group: iGEM22_XJTLU-CHINA (2022-09-18)
CsgA-SmtA
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 561
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 561
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 561
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 561
Illegal AgeI site found at 589 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
To improve the independent folding of each polypeptide, GS-linker was added between csgA and SmtA.
Source
The attachment of SmtA to csgA, which is the major curli subunit protein of E.coli, allows SmtA to form MT proteins in the outer membrane of e.coli, thus achieving the adsorption of metal ions in waste water.