Composite

Part:BBa_K4463004:Design

Designed by: Qiaowa Gong   Group: iGEM22_XJTLU-CHINA   (2022-09-18)


CsgA-SmtA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 561
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 561
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 561
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 561
    Illegal AgeI site found at 589
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To improve the independent folding of each polypeptide, GS-linker was added between csgA and SmtA.


Source

The attachment of SmtA to csgA, which is the major curli subunit protein of E.coli, allows SmtA to form MT proteins in the outer membrane of e.coli, thus achieving the adsorption of metal ions in waste water.

References